Recent analysis using mitochondrial DNA confirmed remains found under a parking lot were those of Richard III.  

Analysis of the y-chromosome DNA indicated that there is at least one break in male DNA, 
indicating that at least one maternal infidelity occurred. Somewhere.

Here are the lines used to glean male line DNA to analyze the remains of Richard III:

Selected Lines of Descendancy from
King Edward III of England used to evaluate
male line DNA of Richard III

1 King Edward III of England (1312 - 1377)
+ Philippa de Hainault (1314 - 1369)
..2 Sir John of Gaunt (1340 - 1399)
..+ Katherine de Roet (1350 - 1403)
....3 Sir John Beaufort (1372 - 1410)
....+ Margaret de Holand (1385 - 1429)
......4 Sir John Beaufort (1404 - 1444)
......+ Margaret de Beauchamp (1412 - 1482)
........5 Margaret Beaufort (1443 - 1509)
........+ Sir Edmund Tudor (1430 - 1456)
..........6 King Henry VII of England (1457 - 1509)(Line of Descent to Queen Elizabeth II)
......4 Sir Edmund Beaufort (1406 - 1455)
......+ Alianor Beauchamp (1407 - 1467)
........5 Sir Henry Beaufort (1436 - 1464)
........+ Jane Hill
..........6 Sir Charles Somerset (1460 - 1526)
..........+ Elizabeth Herbert (1476 - 1509)
............7 Sir Henry Somerset (- 1549)
............+ Elizabeth Browne *
..............8 Sir William Somerset (1527 - 1589)
..............+ Christian North (- 1563)
................9 Sir Edward Somerset (1550 - 1628)
................+ Elizabeth Hastings (1546 - 1621) **********
..................10 Sir Henry Somerset (1577 - 1646)
..................+ Anne Russell (- 1639)
....................11 Sir Edward Somerset (1600 - 1667)
....................+ Elizabeth Dormer (- 1635) *****
......................12 Sir Henry Somerset (1629 - 1700)
......................+ Mary Capell (1630 - 1715) ******
........................13 Sir Charles Somerset (1660 - 1698)
........................+ Rebecca Child (- 1712)
..........................14 Sir Henry Somerset (1684 - 1714)
..........................+ Rachel Noel (- 1709) *****************
............................15 Sir Charles Noel Somerset (1709 - 1756)
............................+ Elizabeth Berkeley (1719 - 1799) *
..............................16 Sir Henry Somerset (1744 - 1803)
..............................+ Elizabeth Boscawen (- 1828)
................................17 William George Henry Somerset (1784 - 1851)
................................+ Elizabeth Molyneux (1790 - 1843)
..................................18 William Somerset (1822 - 1902)
..................................+ Georgianna Amelia Darling (1825 - 1896)
....................................19 Charles Edward Henry Somerset (1862 - 1939)
....................................+ Edith Morten Weatherly (1878 - 1932)
......................................20 William Raglan Henry Guy Somerset (1912 - 1981)
......................................+ Edna Margaret Drybrough-Smith (1912 - 1991)
........................................21 William Michael John Charles Somerset (1934 -)
........................................+ Constance Langley
..........................................22 John Reginald Somerset (1970 -)
..................................18 Col. Fitzroy Molyneux Henry Somerset (1823 - 1901)
..................................+ Jemima Drummond Nairne (1825 - 1874)
....................................19 Arthur William FitzRoy Somerset (1855 - 1937)
....................................+ Gwendolin Adelaide Katherine Georgiana Matilda Somerset
(- 1942)(39*)
......................................20 Arthur Plantagenet Francis Cecil Somerset (1889 - 1957)
......................................+ Mary Frances Elizabeth Randolph (- 1993)
........................................21 John FitzRoy Pechell Somerset (1923 -)
..................................18 Rev. Boscawen Thomas George Henry Somerset (1833 - 1893)
..................................+ Sophia Vernon Powys (1844 - 1935)
....................................19 William Horace Boscawen Somerset (1880 - 1946)
....................................+ Rubie Drummond-Nairne Warner (1886 - 1977)
......................................20 FitzRoy Douglas Boscawen Somerset (1923 -)
......................................+ Sheila Jean Wilson
........................................21 Dr. Douglas William Boscawen Somerset (1965 -)
..2 Edmund Plantagenet (1344 - 1402)
..+ Isabella de Castile (1355 - 1393)
....3 Sir Richard Plantagenet (1376 - 1415)
....+ Anne Mortimer (1390 - 1411)
......4 Sir Richard Plantagenet (1411 - 1460)
......+ Cecily Neville (1415 - 1495)*
........5 King Richard III of England (1452 - 1485)

Asterisks indicate that the wife also has line(s) from Edward III.